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Evaluation of Commercial Universal rRNA Gene PCR Plus Sequencing Tests for Identification of Bacteria and Fungi in Infectious Endocarditis
C Kuhn, C Disque, H Muhl, P Orszag... - Journal of Clinical ..., 2011 - Am Soc Microbiol1 Evaluation of Commercial Universal r RNA Gene PCR Plus Sequencing Tests 1 for Identification
of Bacteria and Fungi in Infectious Endocarditis 2 Running Title: Universal PCR diagnosis of
infectious endocarditis 3 4 ... from 30 patients with suspected infectious endocarditis (IE). ...
Two new commercially available universal r DNA PCR plus sequencing tests, Sepsi Test™ and UMD™ (Molzym, Bremen, Germany), were evaluated using blood specimens and heart valves from 30 patients with suspected infectious endocarditis (IE). The sensitivity of PCR (85%) was nearly twice as high as culture (45%) which in 10/20 IE cases presumably stayed negative as a consequence of growth inhibition of the pathogens by antibiotics. Further, PCR provided the basis for re-classification of 5/10 non-IE into IE cases. Culture-negative infections were identified by PCR, including single infections due to streptococci, Gram-negative bacteria (Escherichia coli, Haemophilus parainfluenzae) and mixed infections involving two Gram-positive bacteria or Candida spp. with Gram-positives. The new commercial tests proved of value for the rapid diagnosis of IE, particularly in cases of culture-negative infections. Issues regarding the feasibility of these tests for routine use are discussed.
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Evaluation of Commercial Universal rRNA Gene PCR Plus Sequencing Tests for Identification of Bacteria and Fungi in Infectious Endocarditis |
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