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Expression of Crip2, a LIM-domain-only protein, in the mouse cardiovascular system under physiological and pathological conditions
TC Wei, HY Lin, CC Lu, CM Chen... - Gene Expression Patterns, 2011 - Elsevier LIM domain-containing proteins mediate proteinprotein interactions and play regulatory roles in various physiopathological processes. The m RNA of Crip2, a LIM-only gene, has been detected abundantly in developing and adult hearts but its cell-type specific ...
Fig. 2. Expression and localization of Crip2 in the developing heart. (A) Immunofluorescence on E8.5 section using an antibody for Crip2. Early expression of Crip2 (green) is observed in the cardiogenic progenitor cells (arrow) of the E8.5 heart. The expression of Crip2 in main aortic arteries is also evident (arrowhead), which is colocalized with endothelial cell marker endoglin (red, inset). (B) A transverse section of an E9.5 embryo. Crip2 is highly expressed in vascular endothelial cells (arrowheads) and the myocardium and endocardium of the heart, while moderate Crip2-positive signal is observed in neuroepithelium (N) of neural tube. (C-E) Crip2 expression at E10.5 embryo. The persistent Crip2 expression is observed in the myocardium and endocardium of the heart in outflow tract (OFT) region (C). High magnification view of boxed area in C is shown in D. In the atrioventricular cushion (AVC), Crip2 is observed in the endocardium, while a weak signal is present in cushion mesenchymes (arrow, E). The expression of Crip2 is also detected in the endothelial cells of the dorsal aorta and cardinal vein at the cervical level. Note that Crip2 expression is also observed in neuroepithelium (N) of neural tube. (F H ) Immunohistochemical detection of Crip2 and cardiac markers in the E11.5 heart. (F F ) Dual immunofluorescent staining was performed to verify the colocalization of Crip2 (green) and cardiac troponin T (c Tn T, red) in the myocardium. (G G ) Crip2 expression is evident in the endocardium where endoglin (red) is co-expressed. (H H ) Expression of Crip2 is absent in the epicardium which is positively labeled by the marker Wt1 (red). Nuclei were counterstained with DAPI (blue). A, atrium; Ao, aorta; AS, aortic sac; N, neuroepithelium; NT, neural tube; OFT, outflow tract; V, ventricle. Scale bar = 100 μm.
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Expression of Crip2, a LIM-domain-only protein, in the mouse cardiovascular system under physiological and pathological conditions |
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