Heart Care Info - Heart Disease Prevention & Treatment

Oncostatin M is expressed in atherosclerotic lesions: A role for Oncostatin M in the pathogenesis of atherosclerosis.

A Albasanz-Puig, J Murray, M Preusch, D Coan... - Atherosclerosis, 2011 - Elsevier OBJECTIVE: Chronic inflammation plays a pivotal role in the development and progression of atherosclerosis. The inflammatory response is mediated by cytokines. The aim of this study was to determine if Oncostatin M (OSM), a monocyte and T-lymphocyte specific ...

Fig. 5. Regulation of OSM-induced SMC proliferation, migration and c FN synthesis by STAT-1 and STAT-3. (A) Proliferation assay: SM Cs were infected with control sh RNA, STAT-1 sh RNA or STAT-3 sh RNA for 24 h. Cells were then incubated in serum-free medium in the presence of OSM (10 ng/ml) and PDGF-BB (50 ng/ml) for 72 h followed by assaying for cell proliferation. **P < 0.01, ***P < 0.001 compared with control sh RNA. (B) Migration assay: SM Cs were infected with control sh RNA, STAT-1 sh RNA or STAT-3 sh RNA for 24 h followed by treatment with OSM (10 ng/ml) in serum-free medium for a further 48 h. Cells were then placed in upper layer of Chemo Tx membranes. PDGF-BB (50 ng/ml) was added to the lower chamber. After 6 h, migrated SM Cs were detected as described in Section 2. Data presented as mean SEM of three experiments performed in triplicate. **P < 0.01 compared with control sh RNA. (C) Analysis of c FN m RNA synthesis: SM Cs were infected with control sh RNA, STAT-1 sh RNA or STAT-3 sh RNA for 24 h. Cells were incubated for 48 h in serum-free medium, followed by stimulation with OSM (10 ng/ml) for 24 h. EDA and EDB m RNA was analyzed by q PCR. Data presented are means SEM for three independent experiments analyzed in duplicate. ***P < 0.001 compared with OSM stimulated cells infected with control sh RNA. (D, E) Analysis of c FN protein synthesis: SM Cs were infected with control sh RNA, STAT-1 sh RNA or STAT-3 sh RNA for 24 h. Cells were incubated for 48 h in serum-free medium, followed by stimulation with OSM (10 ng/ml) for 24 h. EDA and EDB protein synthesis was analyzed by Western blotting. Data shown is representative of 2 experiments.