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IgM phosphorylcholine antibodies inhibit cell death and constitute a strong protection marker for atherosclerosis development, particularly in combination with other ...
R Fiskesund, J Su, I Bulatovic, M Vikstrm... - Results in ..., 2012 - Elsevier Background We have reported that anti-phosphorylcholine (anti-PC) Ig M is a protection marker for human cardiovascular disease (CVD) and atherosclerosis. We here investigate the anti-PC autoantibodies in a well-defined cohort with regard to idiotype, atherosclerosis ...
We have reported that anti-phosphorylcholine (anti-PC) Ig M is a protection marker for human cardiovascular disease (CVD) and atherosclerosis. We here investigate the anti-PC autoantibodies in a well-defined cohort with regard to idiotype, atherosclerosis progression and mechanisms for its protective action.
Serum levels and binding specificities of different anti-PC isotypes were determined in 226 hypertensive individuals enrolled in European Lacidipine Study on Atherosclerosis using ELISA. The mean of the maximum Intima-Media Thicknesses (IMT) in the far walls of common carotids and bifurcations was assessed at the time of inclusion, and four years afterwards. Apoptosis in immune cells was induced with lysophosphatidylcholine (LPC) and quantified using the MTT-assay.
Anti-PC Ig M, Ig A and Ig G1 (but not Ig G2) was negatively associated with IMT-progression. Combining anti-PC Ig M with data on antibodies against oxidized- and malondialdehyde-modified LDL further strengthened this association. At very high levels, anti-PC Ig M exhibited a striking negative association with atherosclerosis progression (OR 0.05; CI 0.006 0.40). Analysis of serum samples taken four years apart in study participants affirmed the stability of anti-PC Ig M titers over time. Examination of fine specificities revealed that the protective isotypes (Ig M, Ig A and Ig G1) are of the Group I idiotype whereas the non-protective Ig G2 subclass was Group II. Anti-PC Ig M inhibited LPC-induced cell death of immune cells.
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IgM phosphorylcholine antibodies inhibit cell death and constitute a strong protection marker for atherosclerosis development, particularly in combination with other ... |
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