Heart Care Info - Heart Disease Prevention & Treatment

FoxO4 inhibits atherosclerosis through its function in bone marrow derived cells.

M Zhu, QJ Zhang, L Wang, H Li... - Atherosclerosis, 2011 - Elsevier Abstract Objectives: Fox O proteins are transcription factors involved in varieties of cellular processes, including immune cell homeostasis, cytokine production, anti-oxidative stress, and cell proliferation and differentiation. Although these processes are implicated in the ...

Fig. 3. Foxo4-deficient B Ms are sufficient to promote enhanced atherosclerosis. Female WT C57B/6 mice were irradiated one day before the BMT. The BM suspension (2 106) from either control WT or Foxo4 / mice was injected intravenously via the tail vein. After 4 weeks recovery, the chimeric mice were fed HFD for four months and sacrificed. (A) PCR genotyping of bone marrows of wild type (lane 1), Foxo4-null (lane 2), and chimeric mice received with Foxo4+/+ bone marrows (lanes 3 6), and Foxo4 / bone marrows (lanes, 7 11). The peripheral blood DNA from Foxo4 / WT mice has the presence of donor Foxo4 / genotype with negligible host DNA remaining. (B) Representative photographs of plaques in the aortic root of chimeric mice received with Foxo4-null (right) and wild type bone marrows. (C) The atherosclerotic lesion in chimeric mice reconstituted with Foxo4 / B Ms is significantly larger than that of mice received with wild type B Ms. Values are mean SEM, p < 0.05. (D) Macrophage contents in the lesions of chimeric mice were quantified using anti-CD68 antibody. (E) Chimeric mice transplanted with Foxo4-null bone marrows have higher number of macrophages than those received with wild type bone marrows after fed with HFD for 4 months. Values are mean SEM, p < 0.05.