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Alteration of volume-regulated chloride channel during macrophage-derived foam cell formation in atherosclerosis
L Hong, ZZ Xie, YH Du, YB Tang, J Tao, XF Lv... - Atherosclerosis, 2011 - Elsevier Methods and ResultsTreatment of RAW264.7 cells with ox-LDL increased intracellular cholesterol
content as well as cell volume. After ox-LDL treatment, the resting [Cl - ] i in isotonic solution was
decreased. Hypotonic solution reduced [Cl - ] i and evoked volume-regulated Cl - current ...
Fig. 2. Effect of ox-LDL on intracellular Cl concentration ([Cl ]i) and volume-regulated Cl movement. (A) Time-dependent effect of 80 μg/m L ox-LDL on Cl movement. i) Representative traces of [Cl ]i under isotonic and hypotonic solution in RAW264.7 macrophages incubated with or without ox-LDL for 6, 12, 24 and 48 h. ii) Alteration of [Cl ]i under isotonic and hypotonic solution in RAW264.7 cells after treatment with ox-LDL for 6 to 48 h. iii) The percentage alteration of [Cl ]i (Δ[Cl ]i,hypo(%)) by hypotonic solution in RAW264.7 cells after treatment with ox-LDL for 6 to 48 h. (*p < 0.05, **p < 0.01 vs control, n = 6 8). (B) Concentration-dependent effect of ox-LDL on Cl movement. i) Alteration of [Cl ]i under isotonic and hypotonic solution in RAW264.7 cells after treatment with 0, 40, 80, or 120 μg/m L ox-LDL for 48 h. ii) The percentage alteration of [Cl ]i (Δ[Cl ]i,hypo(%)) by hypotonic solution in RAW264.7 cells after treatment with 0, 40, 80, or 120 μg/m L ox-LDL for 48 h. (*p < 0.05, **p < 0.01 vs control, n = 8).
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Alteration of volume-regulated chloride channel during macrophage-derived foam cell formation in atherosclerosis |
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